Indirect organogenesis in milkweed (Calotropis procera) from mature zygotic embryo explants. barometz gametophytes were ½ MS with or without 2 mg L -1 BAP. The two best spore culture media for rapid spore germination and development of C. Young heart-shape gametophytes consisting of 110-240 cells were formed in 45-61 days after sowing. Spore germination started 7-14 days after sowing. barometz was observed to be Vittaria-type, and its prothallial development was Drynaria-type. A factorial combination of four BAP concentrations (0, 2, 4, and 6 mg L -1) with four concentrations of NAA (0 0.01 0.03 and 0.05 mg L -1) created 16 treatments replicated in a Completely Randomized Design. The sterilized spores were sown in half-strength Murashige & Skoog (½MS) basal medium supplemented with combinations of 6-Benzylaminopurine (BAP) and ?-Naphthalene acetic acid (NAA). barometz, and (ii) to determine the best culture medium for rapid spore germination and early development of the gametophytes. This study’s objectives were: (i) to observe in vitro spore germination and early gametophyte development of C. In vitro culture is one of the technologies used for ex-situ propagation and conservation of rare and endangered ferns and lycophytes. Globally, populations of this species are under significant pressure due to overexploitation in the wild. It is an important export commodity for traditional and modern medicine. is known as the golden chicken fern and included in Appendix II of CITES. In vitro spore germination and early gametophyte development of Cibotium barometz (L.) J.
0 kommentar(er)
